Abstract
We demonstrate a novel magnetophoretic immunoassay of allergen-specific immunoglobulin E (IgE) based on the magnetophoretic deflection velocity of a microbead that is proportional to the associated magnetic nanoparticles under enhanced magnetic field gradient in a microchannel. In this detection scheme, two types of house dust mites, Dermatophagoides farinae (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus), were used as the model allergens. Polystyrene microbeads were conjugated with each of the mite extracts followed by incubation with serum samples. The resulting mixture was then reacted with magnetic nanoparticle-conjugated anti-human IgE for detection of allergen-specific IgE by using sandwich immuno-reactions. A ferromagnetic microstructure combined with a permanent magnet was employed to increase the magnetic field gradient (∼104 T/m) in a microfluidic device. The magnetophoretic velocities of microbeads were measured in a microchannel under applied magnetic field, and the averaged velocity was well correlated with the concentration of allergen-specific IgE in serum. From the analysis of pooled sera obtained from 44 patients, the detection limits of the allergen-specific human IgEs for D. farinae and D. pteronyssinus were determined to be 565 (0.045 IU/mL) and 268 fM (0.021 IU/mL), respectively. These values are 1 order of magnitude lower than those by a conventional CAP system. For evaluation of reproducibility and accuracy, unknown sera were subjected to a blind test by using the developed assay system, and they were compared with the CAP system. As a result, coefficient of variance was less than 10%, and the developed method enabled a fast assay with a tiny amount of serum (∼10 μL).
| Original language | English |
|---|---|
| Pages (from-to) | 2214-2220 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 79 |
| Issue number | 6 |
| DOIs | |
| State | Published - 15 Mar 2007 |
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Hahn, Y. K., Jin, Z., Kang, J. H., Oh, E., Han, M. K., Kim, H. S., Jang, J. T., Lee, J. H., Cheon, J., Kim, S. H., Park, H. S. (2007). Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient. Analytical Chemistry, 79(6), 2214-2220. https://doi.org/10.1021/ac061522l
Hahn, Young Ki ; Jin, Zongwen ; Kang, Joo H. et al. / Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient. In: Analytical Chemistry. 2007 ; Vol. 79, No. 6. pp. 2214-2220.
@article{c3fecf30720143a2acb309b3aa04ab8b,
title = "Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient",
abstract = "We demonstrate a novel magnetophoretic immunoassay of allergen-specific immunoglobulin E (IgE) based on the magnetophoretic deflection velocity of a microbead that is proportional to the associated magnetic nanoparticles under enhanced magnetic field gradient in a microchannel. In this detection scheme, two types of house dust mites, Dermatophagoides farinae (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus), were used as the model allergens. Polystyrene microbeads were conjugated with each of the mite extracts followed by incubation with serum samples. The resulting mixture was then reacted with magnetic nanoparticle-conjugated anti-human IgE for detection of allergen-specific IgE by using sandwich immuno-reactions. A ferromagnetic microstructure combined with a permanent magnet was employed to increase the magnetic field gradient (∼104 T/m) in a microfluidic device. The magnetophoretic velocities of microbeads were measured in a microchannel under applied magnetic field, and the averaged velocity was well correlated with the concentration of allergen-specific IgE in serum. From the analysis of pooled sera obtained from 44 patients, the detection limits of the allergen-specific human IgEs for D. farinae and D. pteronyssinus were determined to be 565 (0.045 IU/mL) and 268 fM (0.021 IU/mL), respectively. These values are 1 order of magnitude lower than those by a conventional CAP system. For evaluation of reproducibility and accuracy, unknown sera were subjected to a blind test by using the developed assay system, and they were compared with the CAP system. As a result, coefficient of variance was less than 10%, and the developed method enabled a fast assay with a tiny amount of serum (∼10 μL).",
author = "Hahn, {Young Ki} and Zongwen Jin and Kang, {Joo H.} and Eunkeu Oh and Han, {Min Kyu} and Kim, {Hak Sung} and Jang, {Jung Tak} and Lee, {Jae Hyun} and Jinwoo Cheon and Kim, {Seung Hyun} and Park, {Hae Sim} and Park, {Je Kyun}",
year = "2007",
month = mar,
day = "15",
doi = "10.1021/ac061522l",
language = "English",
volume = "79",
pages = "2214--2220",
journal = "Analytical Chemistry",
issn = "0003-2700",
number = "6",
}
Hahn, YK, Jin, Z, Kang, JH, Oh, E, Han, MK, Kim, HS, Jang, JT, Lee, JH, Cheon, J, Kim, SH, Park, HS 2007, 'Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient', Analytical Chemistry, vol. 79, no. 6, pp. 2214-2220. https://doi.org/10.1021/ac061522l
Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient. / Hahn, Young Ki; Jin, Zongwen; Kang, Joo H. et al.
In: Analytical Chemistry, Vol. 79, No. 6, 15.03.2007, p. 2214-2220.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient
AU - Hahn, Young Ki
AU - Jin, Zongwen
AU - Kang, Joo H.
AU - Oh, Eunkeu
AU - Han, Min Kyu
AU - Kim, Hak Sung
AU - Jang, Jung Tak
AU - Lee, Jae Hyun
AU - Cheon, Jinwoo
AU - Kim, Seung Hyun
AU - Park, Hae Sim
AU - Park, Je Kyun
PY - 2007/3/15
Y1 - 2007/3/15
N2 - We demonstrate a novel magnetophoretic immunoassay of allergen-specific immunoglobulin E (IgE) based on the magnetophoretic deflection velocity of a microbead that is proportional to the associated magnetic nanoparticles under enhanced magnetic field gradient in a microchannel. In this detection scheme, two types of house dust mites, Dermatophagoides farinae (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus), were used as the model allergens. Polystyrene microbeads were conjugated with each of the mite extracts followed by incubation with serum samples. The resulting mixture was then reacted with magnetic nanoparticle-conjugated anti-human IgE for detection of allergen-specific IgE by using sandwich immuno-reactions. A ferromagnetic microstructure combined with a permanent magnet was employed to increase the magnetic field gradient (∼104 T/m) in a microfluidic device. The magnetophoretic velocities of microbeads were measured in a microchannel under applied magnetic field, and the averaged velocity was well correlated with the concentration of allergen-specific IgE in serum. From the analysis of pooled sera obtained from 44 patients, the detection limits of the allergen-specific human IgEs for D. farinae and D. pteronyssinus were determined to be 565 (0.045 IU/mL) and 268 fM (0.021 IU/mL), respectively. These values are 1 order of magnitude lower than those by a conventional CAP system. For evaluation of reproducibility and accuracy, unknown sera were subjected to a blind test by using the developed assay system, and they were compared with the CAP system. As a result, coefficient of variance was less than 10%, and the developed method enabled a fast assay with a tiny amount of serum (∼10 μL).
AB - We demonstrate a novel magnetophoretic immunoassay of allergen-specific immunoglobulin E (IgE) based on the magnetophoretic deflection velocity of a microbead that is proportional to the associated magnetic nanoparticles under enhanced magnetic field gradient in a microchannel. In this detection scheme, two types of house dust mites, Dermatophagoides farinae (D. farinae) and Dermatophagoides pteronyssinus (D. pteronyssinus), were used as the model allergens. Polystyrene microbeads were conjugated with each of the mite extracts followed by incubation with serum samples. The resulting mixture was then reacted with magnetic nanoparticle-conjugated anti-human IgE for detection of allergen-specific IgE by using sandwich immuno-reactions. A ferromagnetic microstructure combined with a permanent magnet was employed to increase the magnetic field gradient (∼104 T/m) in a microfluidic device. The magnetophoretic velocities of microbeads were measured in a microchannel under applied magnetic field, and the averaged velocity was well correlated with the concentration of allergen-specific IgE in serum. From the analysis of pooled sera obtained from 44 patients, the detection limits of the allergen-specific human IgEs for D. farinae and D. pteronyssinus were determined to be 565 (0.045 IU/mL) and 268 fM (0.021 IU/mL), respectively. These values are 1 order of magnitude lower than those by a conventional CAP system. For evaluation of reproducibility and accuracy, unknown sera were subjected to a blind test by using the developed assay system, and they were compared with the CAP system. As a result, coefficient of variance was less than 10%, and the developed method enabled a fast assay with a tiny amount of serum (∼10 μL).
UR - http://www.scopus.com/inward/record.url?scp=33947412001&partnerID=8YFLogxK
U2 - 10.1021/ac061522l
DO - 10.1021/ac061522l
M3 - Article
C2 - 17288405
AN - SCOPUS:33947412001
SN - 0003-2700
VL - 79
SP - 2214
EP - 2220
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 6
ER -
Hahn YK, Jin Z, Kang JH, Oh E, Han MK, Kim HS et al. Magnetophoretic immunoassay of allergen-specific IgE in an enhanced magnetic field gradient. Analytical Chemistry. 2007 Mar 15;79(6):2214-2220. doi: 10.1021/ac061522l
