What are the tools used in molecular biology?

Molecular Biology Tools cDNA clones. Cloning tools. CRISPR/Cas9 Technology. DNA arrays. Electrophoresis products. Next Generation Sequencing (NGS) Nucleic acids extraction. PCR products. More items...

What is Neb Cutter in bioinformatics?

NEBcutter -- A restriction analysis tool Find restriction digestion map of a DNA sequence. Highlights: This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.

What is the most common technique used in molecular biology?

Most important techniques are Polymerase Chain Reaction (PCR) , Expression cloning, Gel electrophoresis, Macromolecule blotting and probing, Arrays (DNA array and protein array).

What are the four types of molecular biology?

There are four major classes of biological macromolecules ( carbohydrates, lipids, proteins, and nucleic acids ), and each is an important component of the cell and performs a wide array of functions.

What is NEB in molecular biology?

Founded in the mid-1970s as a collective of scientists committed to developing innovative products for the life sciences industry, New England Biolabs is proud to be a world leader in the discovery and production of enzymes for molecular biology applications .

What does NEB buffer do?

NEB's restriction enzyme buffer system makes your restriction digests easy and convenient . We are able to offer >210 restriction enzymes that cut in a single buffer, rCutSmart™ . This improves ease-of-use, especially when performing double digests.

What are zero cutters?

Note 2: 0 cutters are useful for engineering unique restriction sites into DNA molecules . First ensure that the site does not occur in the plasmid construct. Then introduce the restriction site flanking the region of DNA to be removed.

What apparatus is used in molecular biology?

Standard equipment used in the molecular biology laboratory includes pipettes, centrifuges , as well as other tools that will be introduced in future lab exercises.

What are the tools used in molecular taxonomy?

DNA barcoding. DNA hybridization. DNA Finger printing. Polymerase chain Reaction (PCR) Restriction Fragment Length Polymorphisms analysis (RELP). Jun 14, 2020

What tools are used to study biology?

Basic Equipment Used in Biology Experiments Microscopes. Biologists use microscopes of differing powers to see organisms and samples more closely. ... Slides, test tubes, and petri dishes. ... Dyes and other indicators. ... Forceps, probes, and scalpels. ... Beakers, flasks, and Bunsen burners. Mar 26, 2016

What are some biological tools?

This document outlines several modern biological techniques used in laboratories, including cell and tissue culture, centrifugation, chromatography, gel electrophoresis, spectrophotometry, polymerase chain reaction (PCR), DNA sequencing, immunoassays, DNA cloning, and microarrays.

Molecular Biology Research Tools

HomeResourcesInteractive Tools

Product Selection

Competitor Cross-Reference Tools
Use this tool to select another company's product and find out which NEB product is compatible. Choose either the competitor’s product name or catalog number from the available selections, and this tool will identify the recommended NEB product.
NEBcloner®
Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.
NEBNext Selector
Use this tool to guide you through selection of NEBNext reagents for next generation sequencing sample preparation.
PCR Selector
Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.
Exo Selector
Use this tool to simplify the process of selecting the appropriate exonucleases for use in your nucleic acid digestion workflows. The tool guides you to product recommendations based on your answers to a few simple questions.
PCR Fidelity Estimator
Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.

Restriction Enzyme Tools

Double Digest Finder
Part of NEBcloner, this tool will guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.
Enzyme Finder
Use this tool to select restriction enzymes by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.
NEBcloner®
Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.
NEBcutter™ v3.0
Use this tool to identify the restriction sites, indicate cut frequency and methylation state sensitivity within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. New features added with v3.0.

Primer Design Tools

NEBuilder® Assembly Tool
NEBuilder Assembly Tool can be used to design primers for your NEBuilder® HiFi DNA Assembly or Gibson Assembly® reactions, based on the entered fragment sequences and the polymerase being used for amplification.
NEBridge® Golden Gate Assembly Tool
Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.
NEB LAMP Primer Design Tool
NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. Fixed primers can be specified for the design of LAMP primers, and subsequent Loop primers are then designed based on LAMP primer selection.
NEBaseChanger
NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5 Site-Directed Mutagenesis Kit. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.

Experimental Design

Glycan Analyzer
Use this tool to interpret Ultra or High Pressure liquid chromatography (UPLC/HPLC) N-glycan profiles following exoglycosidase digestions.
NEBcloner®
Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.
DNA Sequences and Maps Tool
Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.
EnGen sgRNA Template Oligo Designer
EnGen sgRNA Template Oligo Designer can be used to design target-specific DNA oligos for use with the EnGen sgRNA Synthesis Kit, S. pyogenes (NEB #E3322).
NEBNext Custom RNA Depletion Design Tool
This tool designs probes to be used with the NEBNext RNA Depletion Core Reagent Set or to supplement an existing NEBNext Depletion Kit for the depletion of unwanted RNA species.
PCR Fidelity Estimator
Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.
NEBridge^® Ligase Fidelity Tools
Use this tool to design high fidelity Golden Gate Assemblies under various experimental conditions.

Calculators

NEBioCalculator®
Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Additional features include sgRNA Template Oligo Design and qPCR library quantification.
Thermostable Ligase Reaction Temperature Calculator
This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template.
Read Coverage Calculator
This tool allows for easy calculation of values associated with read coverage in NGS protocols.
Tm Calculator
Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.
NEBuilder^® Protocol Calculator
Use this tool to calculate the optimal amounts of input DNA sequences for the NEBuilder^® HiFi assembly reaction given the length and concentration of each input fragment.

Databases

REBASE®
Use this tool as a guide to the ever-changing landscape of restriction enzymes. REBASE, the Restriction Enzyme DataBASE, is a dynamic, curated database of restriction enzymes and related proteins.
Polbase
Polbase is a repository of biochemical, genetic, and structural information about DNA Polymerases.

Additional Tools

NEBnow Locator
Use this tool to find an NEBNow Freezer Program near you. Search by city, company or institution.
NEBeta™ Tools
View our selection of online tools currently in development. These tools are not yet optimized for design and usability – we are looking for feedback on functionality and utility to improve them for future use.

Molecular Biology Research Tools | NEB (2024)