Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects (2024)

Mortaz, E., Soroush, D., Khalilzadeh, S., Akbar Velayati, A., Garssen, J. (2014). Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects. European Respiratory Journal, 44.

Mortaz, Esmaeil ; Soroush, Dina ; Khalilzadeh, Sohila et al. / Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects. In: European Respiratory Journal. 2014 ; Vol. 44.

@article{867b9ed6c90b4921bdbf9f3799d14a9f,

title = "Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects",

abstract = "Introduction: Tetrameric Ig are produced and secreted by plasma cells. However, it is well documented that plasma cells also produce and secrete Ig-free light chains (FLC; and ). Recent studies in animal models for allergy suggest the existence of an IgE-independent hypersensitivity response involving antigen-induced mast cell activation, mediated by FLCs. In this study we aimed to explore the relation between serum FLC and IgE in various patients with high levels of specific IgE for food and respiratory allergens. Materials and Methods: 20 subjects with food allergy, 21 subjecs with respiratory allergy and 15 controls were selected and serum was collected for determination of kappa and lambda FLCs, total IgE and specific IgE. Total IgE, FLCs were determined by ELISA. Specific food and respiratory allergens was determined by Improvio C Blotting Methods. Results: The results show that FLC are significantly increased in patients with high allergen-specific IgE as detected by immunoblotting (for 30 food and 30 respiratory allergens). Among the food allergens, the highest specific IgE was detected for celery, carrot, cheese mix, and milk and spelt flour, Derm.farinae, Acarus siro, co*ckroach, mixed grasses and rye pollen for the respiratory allergens. Milk allergic subjects show significant higher increases in than FLCs, while in others -for example celery allergics- this augmentation was reverse. No significant association between and FLCs with others was seen. Conclusion: FLC subunits may be considered as unique markers for allergic sensitization.",

keywords = "immunoglobulin E, immunoglobulin light chain, allergen, immunoglobulin, antigen, food allergen, marker, serum, society, European, food, plasma cell, celery, milk, human, patient, flour, hypersensitivity, allergy, animal model, cheese, carrot, light chain, immunoblotting, respiratory tract allergy, food allergy, sensitization, laryngeal mask, Acarus siro, mast cell, pollen, rye, cell activation, Poaceae, co*ckroach, enzyme linked immunosorbent assay",

author = "Esmaeil Mortaz and Dina Soroush and Sohila Khalilzadeh and {Akbar Velayati}, Ali and Johan Garssen and Frank Redegeld",

year = "2014",

month = sep,

day = "1",

language = "English",

volume = "44",

journal = "European Respiratory Journal",

issn = "0903-1936",

publisher = "European Respiratory Society",

}

Mortaz, E, Soroush, D, Khalilzadeh, S, Akbar Velayati, A, Garssen, J 2014, 'Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects', European Respiratory Journal, vol. 44.

Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects. / Mortaz, Esmaeil; Soroush, Dina; Khalilzadeh, Sohila et al.
In: European Respiratory Journal, Vol. 44, 01.09.2014.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects

AU - Mortaz, Esmaeil

AU - Soroush, Dina

AU - Khalilzadeh, Sohila

AU - Akbar Velayati, Ali

AU - Garssen, Johan

AU - Redegeld, Frank

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Introduction: Tetrameric Ig are produced and secreted by plasma cells. However, it is well documented that plasma cells also produce and secrete Ig-free light chains (FLC; and ). Recent studies in animal models for allergy suggest the existence of an IgE-independent hypersensitivity response involving antigen-induced mast cell activation, mediated by FLCs. In this study we aimed to explore the relation between serum FLC and IgE in various patients with high levels of specific IgE for food and respiratory allergens. Materials and Methods: 20 subjects with food allergy, 21 subjecs with respiratory allergy and 15 controls were selected and serum was collected for determination of kappa and lambda FLCs, total IgE and specific IgE. Total IgE, FLCs were determined by ELISA. Specific food and respiratory allergens was determined by Improvio C Blotting Methods. Results: The results show that FLC are significantly increased in patients with high allergen-specific IgE as detected by immunoblotting (for 30 food and 30 respiratory allergens). Among the food allergens, the highest specific IgE was detected for celery, carrot, cheese mix, and milk and spelt flour, Derm.farinae, Acarus siro, co*ckroach, mixed grasses and rye pollen for the respiratory allergens. Milk allergic subjects show significant higher increases in than FLCs, while in others -for example celery allergics- this augmentation was reverse. No significant association between and FLCs with others was seen. Conclusion: FLC subunits may be considered as unique markers for allergic sensitization.

AB - Introduction: Tetrameric Ig are produced and secreted by plasma cells. However, it is well documented that plasma cells also produce and secrete Ig-free light chains (FLC; and ). Recent studies in animal models for allergy suggest the existence of an IgE-independent hypersensitivity response involving antigen-induced mast cell activation, mediated by FLCs. In this study we aimed to explore the relation between serum FLC and IgE in various patients with high levels of specific IgE for food and respiratory allergens. Materials and Methods: 20 subjects with food allergy, 21 subjecs with respiratory allergy and 15 controls were selected and serum was collected for determination of kappa and lambda FLCs, total IgE and specific IgE. Total IgE, FLCs were determined by ELISA. Specific food and respiratory allergens was determined by Improvio C Blotting Methods. Results: The results show that FLC are significantly increased in patients with high allergen-specific IgE as detected by immunoblotting (for 30 food and 30 respiratory allergens). Among the food allergens, the highest specific IgE was detected for celery, carrot, cheese mix, and milk and spelt flour, Derm.farinae, Acarus siro, co*ckroach, mixed grasses and rye pollen for the respiratory allergens. Milk allergic subjects show significant higher increases in than FLCs, while in others -for example celery allergics- this augmentation was reverse. No significant association between and FLCs with others was seen. Conclusion: FLC subunits may be considered as unique markers for allergic sensitization.

KW - immunoglobulin E

KW - immunoglobulin light chain

KW - allergen

KW - immunoglobulin

KW - antigen

KW - food allergen

KW - marker

KW - serum

KW - society

KW - European

KW - food

KW - plasma cell

KW - celery

KW - milk

KW - human

KW - patient

KW - flour

KW - hypersensitivity

KW - allergy

KW - animal model

KW - cheese

KW - carrot

KW - light chain

KW - immunoblotting

KW - respiratory tract allergy

KW - food allergy

KW - sensitization

KW - laryngeal mask

KW - Acarus siro

KW - mast cell

KW - pollen

KW - rye

KW - cell activation

KW - Poaceae

KW - co*ckroach

KW - enzyme linked immunosorbent assay

M3 - Article

SN - 0903-1936

VL - 44

JO - European Respiratory Journal

JF - European Respiratory Journal

ER -

Mortaz E, Soroush D, Khalilzadeh S, Akbar Velayati A, Garssen J, Redegeld F. Late-breaking abstract: Correlation of serum free light chain immunoglobulins with total IgE and food- and respiratory allergen-specific IgE in serum of allergic subjects. European Respiratory Journal. 2014 Sept 1;44.